Principles and practices of genetic engineering and recombinant DNA technology, isolation and purification of DNA and RNA, restriction enzyme, ligation, blotting, hybridization and autoradiography will be reviewed. Other topics will cover cloning in bacteria and eukaryotes, DNA mini-preps and electrophoretic analysis of library colonies. Plants regeneration such as somatic and embryogenesis and organogenesis as well as culture types are also covered. Some broad and transgenic animals and their applications, in vitro fertilization and embryo transfer, cloning and its potential applications will be discussed.