The course will emphasize the construction and analysis of DNA/genomic libraries, preparation of synthetic oligonucleotide probes, purification and radiolabelling of DNA and hybridization. The course also covers also covers DNA amplification using Polymerase Chain Reaction and sequencing of the amplified DNA, Recombinant DNA technology involving site-directed mutagenesis as well as transformation and expression in vectors and hosts will be discussed. Detection and analysis of expressed proteins from cloned genes will also be considered.