This course is a continuation of BCH 212. The main focus is on the use of UV/visible, infrared, fluorimetry, circular dichroism, flame photometry, electron spin resonance, nuclear magnetic resonance, X-ray diffraction, mass spectrometry and microscopy methods. The course also provides basic concepts in the application of radiometric techniques in biochemical research. The course will also focus on detection, purification and identification of macromolecules. Chromatography, homogenisation, centrifugation methods and high-throughput separation systems will be discussed. Other topics to be covered include spectroscopy, polarimetry, titrations, colorimetry, and flame photometry.