The course aims at providing students with theory and the practical knowledge in physical methods in biology. The course begins with the general principles of analytical biochemistry but  the main focus is on the physical characterization of biological macromolecules. Here, the emphasis is on the use of UV/visible, infrared, fluorimetry, circular dichroism, flame photometry, electron spin resonance, nuclear magnetic resonance, X-ray diffraction, mass spectrometry and microscopy methods. The course also provides basic concepts in the application of radiometric techniques in biochemical research.